Rates of Marine Picoplankton Production: A Novel Approach
Brian Binder (Dept of Marine Sciences, Univ of Georgia, Athens, GA, USA)
Georgia Sea Grant College Program (R/AT-4-PD)
6/1/97 - 12/31/99 (complete)
Photosynthetic picoplankton are important primary producers in many marine ecosystems.
Carbon fixed by these picoplankton populations is thought to be primarily available
to higher trophic levels via the microbial food web. Yet the factors regulating
picoplankton populations are poorly understood at present. An important step
for improving our understanding of these factors is the development of facile
methods for measuring the growth rates of the relevant populations in situ.
This project is directed toward developing techniques for assessing the growth
rates of natural picoplankton populations using rRNA-targeted Protein Nucleic
Acid (PNA) probes.
- PNA probe methods
developed for Prochlorococcus and Synechococcus - and applied
to natural samples with excellent results.
- First reported
whole-cell hybridization of Prochlorococcus species.
Worden, A.Z., S.W. Chisholm, and B.J. Binder. 2000. In situ hybridization of
Prochlorococcus and Synechococcus (marine Cyanobacteria) spp.
with rRNA-targeted peptide nucleic acid probes. Appl. Environ. Microbiol. 66:284-289.