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A Study on the Protozoan Pathogens Perkinsus marinus and Haplosporidium nelsoni in the Easter Oyster, Crassostrea virginica within Georgia’s Recreational Shellfish Harvest Areas
PIs: Ellie Covington and Alan Power (Univ of Georgia Marine Extension Service)
Support: University of Georgia Marine Extension Service
Timeframe: Jan 2006 - ongoing (quarterly)
Results to date :
Our study employs the Perkinsus marinus assay protocol as described by Powell and Ellis (1998). This method involves a 14-day incubation period of tissue stored in Ray’s Fluid Thioglycollate Medium (RFTM). After the incubation period the tissue is examined microscopically and assigned an infection intensity rating based on the semiquantitative scale of infection adapted from Mackin (1962) by Craig et al. (1989). This has been the traditional method of Perkinsus marinus detection. It is relatively inexpensive but is labor intensive and subject to user bias when reading slides and my result in false negatives when looking at tissues with very low infection levels. We are therefore investigating a new technique that has been developed for assessing Perkinsus marinus using PCR methodology (Gauthier et al., 2006). While this method is certainly quicker and perhaps more accurate it has the drawback of being much more expensive. We would welcome the opportunity to work with others and to pursue funding to support a long term shellfish disease monitoring program.
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|This page was updated October 13, 2006|